However, cells responsible for mediating innate or natural immunity have been recently recognized to also contribute to anti-tumor defense. T cells that mediate tumor-specific responses are generally considered to be the major anti-tumor effectors. However, it has been difficult to distinguish the part played by individual immune cell subsets in these processes. This unique study design demonstrates that A-NK cells adoptively transferred to the liver via the intraarterial route have preferential access and substantial accumulation to the tumor site.Įffector functions mediated by cells of the immune system are thought to play a crucial role in the control of tumor development and progression. Binding of A-NK cells to liver neoplastic tissues was observed only after i.a. injected A-NK cells was virtually confined to the spleen and liver. When injected i.v., these cells localized to the lung before being visible in the spleen and liver. ResultsĪ-NK cells expressed a donor-dependent CD56 +CD16 +CD3 - (NK) or CD56 +CD16 +CD3 + (NKT) phenotype. Migration of these cells to various organs was evaluated by SPET and their differential localization to normal and neoplastic liver was demonstrated after i.v.
After 30 days, each patient had a new preparation of 111In-A-NK cells injected i.v. in the liver of three colon carcinoma patients. Patients and methodsĪ-NK cells expanded ex-vivo with IL-2 and labeled with 111In-oxine were injected i.a. This study compares localization to liver metastases of autologous A-NK cells administered via the systemic (intravenous, i.v.) versus locoregional (intraarterial, i.a.) routes. A critical issue in therapy of metastatic disease is the optimization of NK cell migration to tumor tissues and their persistence therein. Adherent NK (A-NK) cells constitute a subset of IL-2-stimulated NK cells which show increased expression of integrins and the ability to adhere to solid surface and to migrate, infiltrate, and destroy cancer.
Besides being the effectors of native anti-tumor cytotoxicity, NK cells participate in T-lymphocyte responses by promoting the maturation of dendritic cells (DC).
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